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Edward F. Knol, PhD

1786Current position:

Associate professor and group leader preclinical research at the department of Dermatology/Allergology, University Medical Center Utrecht, Utrecht, The Netherlands.

Training Milestones:
• 1981-1987: Biomedical Sciences, University of Amsterdam. The Netherlands.
• 1987-1991: PhD Study, Central Laboratory of the Bloodtransfusion Service, Amsterdam, The Netherlands
• 1992-1993:  Postdoctoral fellow, Dision of Allergy and Clinical Immunology at the Johns Hopkins Asthma and Allergy Center, Baltimore, MD, USA
• 1993-1997: Postdoc, Central Laboratory of the Bloodtransfusion Service, Amsterdam, The Netherlands
• 1997-: Current position.
• European Academy of Allergology and Clinical Immunology,(EAACI)
• Dutch Society for Immunology
• Netherlands Society for Dermatology and Venereology
• Dutch Society for Allergology
• Dutch Society for Experimental Dermatology  
• Board member Dutch Society of Allergology
• Board member Dutch Society for Experimental Dermatology
• Board member Immunology section, EAACI
• Scientific Secretary for the annual meeting of the EAACI in Amsterdam, June 2004
• Co-founder and co-organizer of de Dutch Research School for Allergology

Current research interests:T cells play an important role in the chronic inflammation in atopic dermatitis. We are interested in the selective activation of these T cells in the circulation, as well as in the affected tissue during active disease. Although many T cell activation patterns are typical for allergic diseases or skin inflammation we focus on the specific mechanisms relevant for atopic diseases.
In the area of food allergy we focus on the immune response at the level of T cells as well as IgE-allergen interaction when tolerance is being established. In our model of cow’s milk allergy our interest is the level in which the spontaneous occurrence of tolerance is regulated. In the more persistent model of peanut allergy we aim at determining at which level  the severity of the allergic responses is determined in children as well as in adults. It is clear that allergen-specific T cell activation as well as allergen-specific IgE/IgG4 binding is not sufficient to explain the clinical outcome.

Selected Publications from >100 peer reviewed publications:

• Kapitein B, Hoekstra MO, Nijhuis EH, Hijnen DJ, Arets HG, Kimpen JL, Knol EF. Gene expression in CD4+ T-cells reflects heterogeneity in infant wheezing phenotypes. Eur Respir J. 2008 Nov;32(5):1203-12.

• Flinterman AE, Knol EF, Lencer DA, Bardina L, den Hartog Jager CF, Lin J, Pasmans SG, Bruijnzeel-Koomen CA, Sampson HA, van Hoffen E, Shreffler WG. Peanut epitopes for IgE and IgG4 in peanut-sensitized children in relation to severity of peanut allergy. J Allergy Clin Immunol. 2008 Mar;121(3):737-743.

• Olszewski MB, Groot AJ, Dastych J, Knol EF. TNF trafficking to human mast cell granules: mature chain-dependent endocytosis. J Immunol. 2007 May 1;178(9):5701-9

• Kapitein B, Tiemessen MM, Liu WM, van Ieperen-van Dijk AG, Hoekstra MO, van Hoffen E, Knol EF. The interleukin-10 inducing effect of transforming growth factor-beta on human naive CD4+ T cells from cord blood is restricted to the TH1 subset. Clin Exp Immunol. 2007 Feb;147(2):352-8.

• Hijnen D, Nijhuis E, Bruin-Weller M, Holstege F, Koerkamp MG, Kok I, Bruijnzeel-Koomen C, Knol EF. Differential expression of genes involved in skin homing, proliferation, and apoptosis in CD4+ T cells of patients with atopic dermatitis. J Invest Dermatol. 2005 Dec;125(6):1149-55.

• Tiemessen MM, Van Ieperen-Van Dijk AG, Bruijnzeel-Koomen CA, Garssen J, Knol EF, Van Hoffen E. Cow's milk-specific T-cell reactivity of children with and without persistent cow's milk allergy: key role for IL-10. J Allergy Clin Immunol. 2004 May;113(5):932-9.

• Hijnen D, De Bruin-Weller M, Oosting B, Lebre C, De Jong E, Bruijnzeel-Koomen C, Knol EF. Serum thymus and activation-regulated chemokine (TARC) and cutaneous T cell-attracting chemokine (CTACK) levels in allergic diseases: TARC and CTACK are disease-specific markers for atopic dermatitis. J Allergy Clin Immunol. 2004 Feb;113(2):334-40.

• Tiemessen MM, Van Hoffen E, Knulst AC, Van Der Zee JA, Knol EF, Taams LS. CD4 CD25 regulatory T cells are not functionally impaired in adult patients with IgE-mediated cow's milk allergy. J Allergy Clin Immunol. 2002 Dec;110(6):934-6.

• Lie WJ, Mul FP, Roos D, Verhoeven AJ, Knol EF. Degranulation of human basophils by picomolar concentrations of IL-3, IL-5, or granulocyte-macrophage colony-stimulating factor. J Allergy Clin Immunol. 1998 May;101(5):683-90.

• Liu L, Mul FP, Lutter R, Roos D, Knol EF. Transmigration of human neutrophils across airway epithelial cell monolayers is preferentially in the physiologic basolateral-to-apical direction. Am J Respir Cell Mol Biol. 1996 Dec;15(6):771-80.

• Knol EF, Tackey F, Tedder TF, Klunk DA, Bickel CA, Sterbinsky SA, Bochner BS. Comparison of human eosinophil and neutrophil adhesion to endothelial cells under nonstatic conditions. Role of L-selectin. J Immunol. 1994 Sep 1;153(5):2161-7.

• Knol EF, Kuijpers TW, Mul FP, Roos D.  Stimulation of human basophils results in homotypic aggregation. A response independent of degranulation. J Immunol. 1993 Nov 1;151(9):4926-33.

• Wagemaker G, van Gils FC, Burger H, Dorssers LC, van Leen RW, Persoon NL, Wielenga JJ, Heeney JL, Knol EF. Highly increased production of bone marrow-derived blood cells by administration of homologous interleukin-3 to rhesus monkeys. Blood. 1990 Dec 1;76(11):2235-41.

• Knol EF, Mul FP, Jansen H, Calafat J, Roos D. Monitoring human basophil activation via CD63 monoclonal antibody 435. J Allergy Clin Immunol. 1991 Sep;88(3 Pt 1):328-38.

• Knol EF, Koenderman L, Mul FP, Verhoeven AJ, Roos D.  Differential activation of human basophils by anti-IgE and formyl-methionyl-leucyl-phenylalanine. Indications for protein kinase C-dependent and -independent activation pathways. Eur J Immunol. 1991 Apr;21(4):881-5.